CHARACTERIZATION OF A FULL-LENGTH CDNA FOR RABBIT FACTOR-X

A 300 bp probe generated by the PCR was derived from rabbit genomic DN A using primers from a highly conserved region of the DNA for human fa ctor X (HFX). The probe was used in northern blot analysis of liver RN A to demonstrate an mRNA species of 1.6 kb for the rabbit factor X and subsequently for isolation and characterization of the cDNA for rabbi t factor X (RFX) from a lambda Zap II cDNA library generated from rabb it liver mRNA. The cDNA contains 22 bases upstream from the 5'-transla tion initiation codon, 1470 nucleotides of open reading frame, a stop codon and a 3' poly(A) tail. The cDNA codes for a 40-residue signal/pr opeptide region, followed by a 447-residue mature protein. The deduced amino acid sequence shows a high degree of homology with the sequence of HFX. Inhibitory peptides derived from interactive sites of HFX for activators, cofactor and substrate exerted degrees of inhibition of R FX activation which showed a dependence on extent of homology with the corresponding regions of RFX. (C) 1997 Elsevier Science Ltd.