ISOLATION AND CHARACTERIZATION OF A GENE ENCODING ALPHA-TUBULIN FROM CANDIDA-ALBICANS

Authors
DALY S YACOUB A DUNDON W MASTROMEI G ISLAM K LORENZETTI R
Citation
S. Daly et al., ISOLATION AND CHARACTERIZATION OF A GENE ENCODING ALPHA-TUBULIN FROM CANDIDA-ALBICANS, Gene, 187(2), 1997, pp. 151-158
Citations number
36
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
187
Issue
2
Year of publication
1997
Pages
151 - 158
Database
ISI
SICI code
0378-1119(1997)187:2<151:IACOAG>2.0.ZU;2-C
Abstract
A gene encoding the alpha-tubulin of Candida albicans has been cloned and characterized. Nucleotide sequence analysis reveals the presence o f an intron within the structural gene and predicts the synthesis of a polypeptide of 448 amino acid residues. Comparison of nucleotide and amino acid sequences with the Saccharomyces cerevisiae alpha-tubulin e ncoding genes shows a 75% homology and about 92% similarity respective ly. In contrast to S. cerevisiae, C. albicans appears to possess only one gene for alpha-tubulin which is able to functionally complement a S. cerevisiae cold-sensitive tub1 mutant.