Jp. Ganiere et al., IN-VITRO KINETICS OF BACTERICIDAL ACTIVIT Y OF A SOLUTION CONTAINING A COMBINATION OF ANTIBIOTICS IN MILK, Recueil de medecine veterinaire, 169(4), 1993, pp. 265-272
The kinetics of bactericidal activity of a combination of neomycin, ba
citracin and tetracycline, recommended in the treatment of bovine mast
itis, is studied in vitro by replacing the usual culture mediums by UH
T semi-skimmed milk. Bactericidal activity is needed against Staphyloc
occus aureus and Streptococcus uberis. The antibiotic concentrations t
ested were the same as those measured in milk after a treatment perfor
med during a pharmacocinetic study. At the concentrations tested the t
etracycline dominated the other antibiotics. Bacitracin and neomycin a
ppeared to have a clear synergistic action and despite the reduction i
n activity linked to the presence of milk, this combination remained e
ffective and often bactericidal. The results obtained show that this m
ethodological can contribute to a better understanding of the in vivo
conditions needed for effective treatment of the mammary gland by a co
mbination of locally administered antibiotics.