FORSKOLIN PHOTOAFFINITY PROBES FOR THE EVALUATION OF TUBULIN BINDING-SITES

Authors
CHAVAN AJ RICHARDSON SK KIM H HALEY BE WATT DS
Citation
Aj. Chavan et al., FORSKOLIN PHOTOAFFINITY PROBES FOR THE EVALUATION OF TUBULIN BINDING-SITES, Bioconjugate chemistry, 4(4), 1993, pp. 268-274
Citations number
24
Categorie Soggetti
Biology,Chemistry
Journal title
Bioconjugate chemistry → ACNP
ISSN journal
10431802
Volume
4
Issue
4
Year of publication
1993
Pages
268 - 274
Database
ISI
SICI code
1043-1802(1993)4:4<268:FPPFTE>2.0.ZU;2-5
Abstract
A photoactive C-7 derivative of forskolin, kolinyl2-(3-azido-5-hydroxy -4-iodophenoxy)acetate, enhanced GTP-promoted tubulin polymerization, preferentially bound the beta-subunit, exhibited competition with the noniodinated version of the probe, and displayed saturation of labelin g consistent with the presence of a forskolin binding site or sites on tubulin.