LABELING OF IMMUNOGLOBULINS WITH BIFUNCTIONAL, SULFHYDRYL-SELECTIVE, AND PHOTOREACTIVE COUMARINS

Authors
BARANOWSKAKORTYLEWICZ J KASSIS AI
Citation
J. Baranowskakortylewicz et Ai. Kassis, LABELING OF IMMUNOGLOBULINS WITH BIFUNCTIONAL, SULFHYDRYL-SELECTIVE, AND PHOTOREACTIVE COUMARINS, Bioconjugate chemistry, 4(4), 1993, pp. 300-304
Citations number
27
Categorie Soggetti
Biology,Chemistry
Journal title
Bioconjugate chemistry → ACNP
ISSN journal
10431802
Volume
4
Issue
4
Year of publication
1993
Pages
300 - 304
Database
ISI
SICI code
1043-1802(1993)4:4<300:LOIWBS>2.0.ZU;2-X
Abstract
4-(Bromomethyl)-6,7-dimethoxy-2-oxo-2H-benzopyran (1a) and 4-(bromomet hyl)-7-methoxy-2-oxo-2H-benzopyran (1b) and their iodo analogs (2) rea cted selectively with the sulfhydryl groups generated in a limited red uction of the hinge disulfide bonds of an immunoglobulin G (IgG) givin g proteins labeled with a fluorescent and/or radioactive moiety. Quant itative alkylation of free thiol groups was obtained with 1 and I-125/ 127-2. Photodimerization of these adducts produced IgG with covalently joined heavy chains.