REVERSE-PHASE THIN-LAYER CHROMATOGRAPHY ASSAY OF VITAMIN-K IN BOVINE LIVER

A cass of hemorrhage of unknown origin was observed in cattle and live r samples were submitted to the Diagnostic Lab for assay of Vitamin K or possible coumarol. After evaluating normal phase and reverse phase Thin Layer Chromatography (TLC) plates and different solvents, reverse phase TLC plate with indicator and methylene chloride:methanol 70:30 were chosen for direct method of detection and indication of Vitamin K . Gas Chromatography and Densitometry were used to quantitate the Vita min K present in liver samples and confirmation of Vitamin K was done by Mass Spectroscopy.