PARTIAL CLONING OF PROHIBITIN CDNA FROM CANINE, FELINE, BOVINE, EQUINE, AND RABBIT LIVER MESSENGER-RNA BY RT-PCR

Prohibitin is the protein which has an inhibitory function in cell gro wth, and its gene is suggested to be one of putative tumor suppressor genes. In this report, we described a partial cloning of prohibitin cD NAs from canine, feline, bovine, equine, and rabbit liver mRNAs by RT- PCR, and their homology analysis. The sequences of these RT-PCR produc ts were compared with each other as well as those reported for human a nd rat. The homology in this region of prohibitin cDNA was approximate ly 90%, and the amino acid sequence of each RT-PCR product shared more than 95% identity. Therefore, it is concluded that all the RT-PCR pro ducts are a part of prohibitin cDNA of each animal.