REVERSED-PHASE LIQUID-CHROMATOGRAPHIC SEPARATION OF SOME MONOSUBSTITUTED PHENOLS WITH CALIX[6]ARENE-RHO-SULFONATE-MODIFIED ELUENTS

The utility of water-soluble calix[6]arene-rho-sulfonate as a mobile p hase additive has been investigated for the reversed phase liquid chro matographic separation of some monosubstituted phenol isomers. Retenti on factors and separation factors for regioisomers of methoxyphenol, a minophenol, and nitrophenol were measured using methanol - water and a cetonitrile - water mobile phases of varying composition containing ca lix[6]arene-rho-sulfonate, and the values were compared with those obt ained using mobile phases containing no additive. It was observed that addition of calix[6]arene-rho-sulfonate to both acetonitrile - water and methanol - water caused a reduction in the retention of the phenol isomers but generally increased the separation between them, thereby improving the overall separation efficiency.