Temperate coliphage P2 and satellite phage P4 have icosahedral capsids
and contractile tails with side tail fibers. Because P4 requires all
the capsid, tail, and lysis genes (late genes) of P2, the genomes of t
hese phages are in constant communication during P4 development. The P
4 genome (11, 624 bp) and the P2 genome (33.8 kb) share homologous cos
sites of 55 bp which are essential for generating 19-bp cohesive ends
but are otherwise dissimilar. P4 turns on the expression of helper ph
age late genes by two mechanisms: derepression of P2 prophage and tran
sactivation of P2 late-gene promoters. P4 also exploits the morphopoie
tic pathway of P2 by controlling the capsid size to fit its smaller ge
nome. The P4 sid gene product is responsible for capsid size determina
tion, and the P2 capsid gene product, gpN, is used to build both sizes
. The P2 capsid contains 420 capsid protein subunits, and P4 contains
240 subunits. The size reduction appears to involve a major change of
the whole hexamer complex. The P4 particles are less stable to heat in
activation, unless their capsids are coated with a P4-encoded decorati
on protein (the psu gene product). P4 uses a small RNA molecule as its
immunity factor. Expression of P4 replication functions is prevented
by premature transcription termination effected by this small RNA mole
cule, which contains a sequence that is complementary to a sequence in
the transcript that it terminates.