S. Oita et So. Yanagi, STIMULATION OF SCHIZOPHYLLUM-COMMUNE FRUIT BODY FORMATION BY INHIBITOR OF MEMBRANE-FUNCTION AND CELL-WALL SYNTHESIS, Bioscience, biotechnology, and biochemistry, 57(8), 1993, pp. 1270-1274
The fruit body formation of Schizophyllum commune was greatly stimulat
ed by the addition of papulacandin B, aculeacin A, gramicidin S, lutei
nizing hormone-releasing hormone (LH-RH), or digitonin to the culture
media. Papulacandin B and aculeacin A inhibit glucan synthase, which w
as demonstrated with S. commune in vitro in this study. The in vitro S
. commune glucan synthase was activated by phospholipids that countera
cted the stimulation of fruit body formation by papulacandin B. LH-RH,
however, did not inhibit the glucan synthase. Digitonin was known to
inhibit chitin synthase, but polyoxin B, a substrate analogue inhibito
r of chitin synthase, did not stimulate the fruit body formation. The
characteristic common to most of these stimulators of fruit body forma
tion is possible induction of structural changes of plasma membranes t
o which the substances can attach with their biphasic nature. These st
imulators of fruit body formation were mostly accompanied by an eviden
t suppression of hyphal growth, independent of phenoloxidase activity,
and not observed in continuous dark or with monokaryotic strains.