GLUCOSYLATION OF VANILLIN BY CULTURED PLANT-CELLS

Vanillin was converted into the corresponding glucoside in suspension- cultured cells of Ceffea arabica. The maximum efficiency of glucosylat ion was 85% within 24 h after the addition of 1 mm vanillin when cultu red in a modified Murashige and Skoog's medium with 5 mum 2,4-dichloro phenoxyacetic acid and 0.5 muM kinetin. The glucoside was identified a s 4-formyl-2-methoxyphenyl-0-beta-D-glucopyranoside by H-1-NMR, C-13-N MR, FAB-MS, and hydrolysis by alpha- and beta-glucosidases. It retaine d the antimutagenic and antimicrobial activities of vanillin.