PRODUCTION OF VARIOUS PHOSPHATIDYLSACCHARIDES BY PHOSPHOLIPASE-D FROMACTINOMADURA SP STRAIN NO 362

Various phosphatidylsaccharides can be produced in good yields by a on e-step transphatidylation reaction catalyzed by the purified phospholi pase D from Actinomadura sp. strain No. 362. With glucose as the sacch aride, the optimal conditions of transphosphatidylation were investiga ted. Several solvents such as diethylether or tert-butanol and several salts such as NaCl or CaCl2 were effective for phosphatidylglucose pr oduction. The optimal concentration of glucose for phosphatidylglucose production was 0.8 part of the water. With optimal conditions, the co nversion rate of egg lecithin to phosphatidylglucose reached about 85% . With this condition of phosphatidylglucose production, dipalmitoylph osphatidylcholine, dimyristoylphosphatidylcholine, soya lecithin, phos phatidylethanolamine, phosphatidylglycerol, dihexadecylphosphatidylcho line, and sphingomyelin were found to be good substrates. Pentoses, he xoses, and heptoses with primary alcohol groups were also found to be good acceptors Of transphosphatidylation.