A MUTANT ALLELE SKT5 AFFECTING PROTOPLAST REGENERATION AND KILLER TOXIN RESISTANCE HAS DOUBLE MUTATIONS IN ITS WILD-TYPE STRUCTURAL GENE INSACCHAROMYCES-CEREVISIAE

Authors
KAWAMOTO S SASAKI T ITAHASHI S HATSUYAMA Y OHNO T
Citation
S. Kawamoto et al., A MUTANT ALLELE SKT5 AFFECTING PROTOPLAST REGENERATION AND KILLER TOXIN RESISTANCE HAS DOUBLE MUTATIONS IN ITS WILD-TYPE STRUCTURAL GENE INSACCHAROMYCES-CEREVISIAE, Bioscience, biotechnology, and biochemistry, 57(8), 1993, pp. 1391-1393
Citations number
8
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
57
Issue
8
Year of publication
1993
Pages
1391 - 1393
Database
ISI
SICI code
0916-8451(1993)57:8<1391:AMASAP>2.0.ZU;2-6
Abstract
Saccharomyces cerevisiae strains carrying the mutant allele skt5 are r esistant to the killer toxin of Kluyveromyces lactis and are defective in protoplast regeneration. The DNA sequence analysis of the cloned m utant skt5 gene showed a nucleotide substitution (causing a glycine-to -glutamic acid substitution) and also a nucleotide insertion (causing a frameshift at the extreme carboxy-terminal region) in the structural region from its wild-type gene.