IDENTIFICATION OF THE GENE ENCODING THE ACTIVATOR OF (R)-2-HYDROXYGLUTARYL-COA DEHYDRATASE FROM ACIDAMINOCOCCUS-FERMENTANS BY GENE-EXPRESSION IN ESCHERICHIA-COLI
K. Bendrat et al., IDENTIFICATION OF THE GENE ENCODING THE ACTIVATOR OF (R)-2-HYDROXYGLUTARYL-COA DEHYDRATASE FROM ACIDAMINOCOCCUS-FERMENTANS BY GENE-EXPRESSION IN ESCHERICHIA-COLI, FEBS letters, 329(3), 1993, pp. 329-331
(R)-2-Hydroxyglutaryl-CoA dehydratase (HGDA/B) from Acidaminococcus fe
rmentans requires an activator protein for activity. This activator (H
GDC) has not yet been purified from its natural source due to its low
concentration combined with an extreme sensitivity towards oxygen. Gen
e expression in Escherichia coli identified an open reading frame (780
bp) as the gene encoding HGDC. Dehydratase activity was stimulated at
least tenfold by cell-free extracts of E coli cells transformed with
a plasmid carrying hgdC. On the chromosome the hgdC gene is located ju
st before hgdA and hgdB.