IMMUNOLOGICAL IDENTIFICATION OF ORGAN-SPECIFIC PROTEINS AND TRANSCRIPTS IN DEVELOPING BARLEY GRAINS

In vitro translated proteins from poly(A+)RNA of immature barley starc hy endosperm and embryos were immunoadsorbed with antibodies raised ag ainst proteins of aleurone layers, starchy endosperm and embryos. Four starchy endosperm and eight embryo specific transcripts were detected . In addition, several mRNAs were restricted to only two of the three tissues. Comparing SDS-PAGE patterns of the in vivo protein extracts a gainst which the antibodies were raised, four aleurone, six starchy en dosperm and four embryo-specific protein bands were detectable. As for the in vitro translated proteins, several in vivo protein bands were here present in only two of the three tissues. Of eight known barley g rain proteins for which antibodies were available, only three were pre sent in developing embryos.