COMPARISON OF 2 TIME-RESOLVED FLUOROIMMUNOASSAYS (TR-FIA), AS APPLIEDTO ESTRIOL IN HUMAN SERUM AND PROGESTERONE IN BOVINE-MILK

We compared two time-resolved fluoroimmunoassay systems for measuring free oestriol in human serum and progesterone in bovine milk. By readi ng the fluorescence of europium complex of sulphophenyl)-1,10-phenanth roline-2,9-dicarboxylic acid in solution, the measuring range is incre ased for both oestriol (10-50000 ng/l instead of 25-50000 ng/l) and fo r progesterone (10-50000 ng/l instead of 25-10000 ng/1). In addition, the interassay coefficients of variation were lowered from 9.5 to 5.7% for oestriol and from 7.5 to 5.4% for progesterone, at the smaller ho rmone concentrations detectable by each method.