J. Dupuy et al., THERMOSTABILITY OF FUMONISIN-B1, A MYCOTOXIN FROM FUSARIUM-MONILIFORME, IN CORN, Applied and environmental microbiology, 59(9), 1993, pp. 2864-2867
Fumonisin B1 (FB1) is a mycotoxin from Fusarium moniliforme that is fr
equently associated with corn. Thermal treatments are used in many pro
cesses concerning this cereal and its derivatives. The thermostability
of this toxin in dry contaminated corn, resulting from F. moniliforme
culture, was studied in different time-temperature combinations. FB1
was quantified by instrumentalized thin-layer chromatography after a t
wo-step sequential development and postchromatographic derivatization
by p-anisaldehyde. The identity of FB1 in extracts, before and after h
eat treatments, was confirmed by high-pressure liquid chromatography.
For each temperature, the natural logarithm of the ratio of resulting
FB1 on initial content (ln C/C0) is linearly correlated to exposure ti
me. The calculated half-lives (L50), corresponding to the 50% value, w
ere 10 min, 38 min, 175 min, and 8 h at 150, 125, 100, and 75-degrees-
C, respectively. There is a linear relationship between calculated L50
s on a logarithmic scale and temperature. Therefore FB1 is not signifi
cantly destroyed by the main drying processes of corn or thermal treat
ments used for its derivatives. Other associated means are required fo
r detoxification.