PRODUCTION OF L-DIHYDROXYPHENYLALANINE IN ESCHERICHIA-COLI WITH THE TYROSINE PHENOL-LYASE GENE CLONED FROM ERWINIA-HERBICOLA

Authors
FOOR F MORIN N BOSTIAN KA
Citation
F. Foor et al., PRODUCTION OF L-DIHYDROXYPHENYLALANINE IN ESCHERICHIA-COLI WITH THE TYROSINE PHENOL-LYASE GENE CLONED FROM ERWINIA-HERBICOLA, Applied and environmental microbiology, 59(9), 1993, pp. 3070-3075
Citations number
20
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
59
Issue
9
Year of publication
1993
Pages
3070 - 3075
Database
ISI
SICI code
0099-2240(1993)59:9<3070:POLIEW>2.0.ZU;2-9
Abstract
The gene (tutA) encoding tyrosine phenol-lyase from Erwinia herbicola was cloned into Escherichia coli, and fusions to the lac and tac promo ters were constructed. The enzyme was expressed at high levels in E. c oli in the presence of isopropyl-beta-D-thiogalactopyranoside or lacto se as an inducer. L-Dihydroxyphenylalanine was synthesized in high yie ld from catechol, pyruvate, and ammonia by induced cells.