COMPARISON AND GENOMIC SIZING OF ESCHERICHIA-COLI O157-H7 ISOLATES BYPULSED-FIELD GEL-ELECTROPHORESIS

Authors
HARSONO KD KASPAR CW LUCHANSKY JB
Citation
Kd. Harsono et al., COMPARISON AND GENOMIC SIZING OF ESCHERICHIA-COLI O157-H7 ISOLATES BYPULSED-FIELD GEL-ELECTROPHORESIS, Applied and environmental microbiology, 59(9), 1993, pp. 3141-3144
Citations number
24
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
59
Issue
9
Year of publication
1993
Pages
3141 - 3144
Database
ISI
SICI code
0099-2240(1993)59:9<3141:CAGSOE>2.0.ZU;2-G
Abstract
Genomic DNAs of Escherichia coli O157:H7 strains isolated from patient s and food samples were analyzed by pulsed-field gel electrophoresis. The rare-cutting endonucleases SfiI and XbaI generated 6 and 10 distin ct genomic profiles, respectively, for the 22 strains analyzed, indica ting that this technique may find application for epidemiologic studie s. Summation of XbaI fragments from five E. coli 0157:H7 strains estim ated the genomic length at ca. 4.7 Mb.