Genetic variation among existing cultivars and in germplasm collection
s is the outcome of selection during evolution and plant breeding. Mut
agenesis offers the plant breeder a chance to tackle unconventional ob
jectives, particularly those that were at a selection disadvantage in
the past. Effective mutagens are available, but the bottleneck is the
effective selection of rare desired variants from large mutagenized po
pulations. Selection methods must be non-destructive. Grain legume mut
ation breeding has already led to improved cultivars with higher yield
, better grain quality, or stronger resistance to pathogens. Many muta
tions affecting nitrogen fixation related traits have also been report
ed. Some could be useful in breeding better cultivars, but the majorit
y are being used to study the factors interacting in the complex proce
ss of symbiotic nitrogen fixation and to improve the strategy for prod
ucing cultivars with better fixation capacity.