Studies have shown significant coronal dye and bacterial leakage follo
wing exposure of sealed root canals to artificial and natural saliva.
The purpose of this study was to determine the time needed for bacteri
a in natural saliva to contaminate the entire length of root canals ob
turated by lateral and vertical condensation techniques. Forty root ca
nals were cleaned and shaped using a step-back technique. Thirty root
canals were obturated with gutta-percha and root canal sealer using ei
ther lateral or vertical condensation techniques. Five root canals wer
e obturated without a root canal sealer and served as positive control
s. After obturation, the coronal 3 mm of five root canals were sealed
with sticky wax and served as negatie controls. The coronal portions o
f the filling materials were placed in contact with human saliva and t
he number of days required for bacteria in saliva to penetrate the ent
ire root canals were determined. No bacterial leakage occurred in the
negative control group. Complete bacterial leakage occurred within 2 d
ays in the positive control group. All root canals were recontaminated
in less than 30 days. No statistical significant difference was found
between the two methods of obturation.