Mjm. Burgering et al., OBSERVATION OF INTER-SUBUNIT NUCLEAR OVERHAUSER EFFECTS IN A DIMERIC PROTEIN - APPLICATION TO THE ARC REPRESSOR, FEBS letters, 330(1), 1993, pp. 105-109
For the structure determination of symmetric protein dimers it is nece
ssary to distinguish between intra- and inter-subunit NOEs. A method i
s presented to measure selectively the inter-subunit NOEs using unifor
m N-15 and C-13 isotope labelling. This is accomplished by doubly filt
ered 2D NOE experiments on mixtures of native protein with isotope-lab
eled protein. The method has been applied to the Arc repressor and all
ows the characterization of virtually all proton-proton NOEs in terms
of their intra- or inter-subunit nature.