IN-VITRO AND IN-VIVO ASSAYS OF ISOPROPANOL FOR MUTAGENICITY

To assess the mutagenic potential of isopropanol, an in vitro Chinese hamster ovary (CHO) cell/HGPRT gene mutation assay and a bone marrow m icronucleus study in mice were conducted. In the CHO/HGPRT assay, conc entration levels ranged from 0.5 to 5.0 mg/ml. No elevated mutant freq uencies attributable to treatment were observed in the test under eith er activated or non-activated conditions. In the micronucleus assay, m ice were injected intraperitoneally (IP) with either 350, 1,173, or 2, 500 mg/kg of isopropanol at constant volumes of 10 ml/kg. No increased incidence of micronuclei was observed in bone marrow polychromatic er ythrocytes (PCEs) harvested at 24, 48, or 72 hr post-dosing. In both a ssays, negative and positive control mutant frequencies were within hi storical control ranges. These results, in conjunction with previously published data, clearly demonstrate that isopropanol is not a mutagen . (C) 1993 Wiley-Liss, Inc.