INVESTIGATION OF ANEUPLOIDY INDUCTION IN MOUSE OOCYTES FOLLOWING EXPOSURE TO VINBLASTINE-SULFATE, PYRIMETHAMINE, DIETHYLSTILBESTROL DIPHOSPHATE, OR CHLORAL HYDRATE
Jb. Mailhes et al., INVESTIGATION OF ANEUPLOIDY INDUCTION IN MOUSE OOCYTES FOLLOWING EXPOSURE TO VINBLASTINE-SULFATE, PYRIMETHAMINE, DIETHYLSTILBESTROL DIPHOSPHATE, OR CHLORAL HYDRATE, Environmental and molecular mutagenesis, 22(2), 1993, pp. 107-114
The various causative and mechanistic phenomena associated with aneupl
oidy induction require considerable investigation to better understand
the etiology of chromosome missegregation. We investigated the potent
ial of vinblastine sulfate, pyrimethamine, diethylstilbestrol diphosph
ate, and chloral hydrate to induce numerical and structural chromosome
changes in female mouse germ cells. Superovulated ICR mice were admin
istered the compounds either by intraperitoneal injection or oral gava
ge, and oocytes were collected and processed for cytogenetic analysis
17 hr later. Vinblastine sulfate, administered i.p., induced a signifi
cant increase in the frequency of ovulated MI oocytes and of hyperploi
d MII oocytes compared to controls, but did not increase the frequency
of structural aberrations. Pyrimethamine, diethylstilbestrol diphosph
ate, and chloral hydrate did not increase the frequency of numerical o
r structural chromosome changes in female mouse germ cells. (C) 1993 W
iley-Liss, Inc.