ISOLATION AND N-TERMINAL SEQUENCE OF 2 LOW-MOLECULAR-WEIGHT CALCIUM-BINDING PROTEINS FROM PIG GRANULOCYTES

1. Two small, abundant calcium-binding proteins were isolated from pig granulocytes. They were named p7A and p7B. Relative molecular masses were approx. 32,000 for p7A and 13,000 for p7B, when obtained by Sepha dex G-75 gel filtration, while it was 7000 for both proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). 2. N-t erminal sequence analysis suggests that p7A is homologous to human and mouse MRP-8 and that p7B may be related to human and mouse MRP-14, th ough some properties of the latter-such as mobility on SDS-PAGE-were f ound to be different. In addition, p7A and p7B could be resolved under native conditions, contrasting with the fact that human and mouse MRP -8/MRP-14 form noncovalent complexes.