O. Gunturkun et al., DIFFERENT SETS OF AFFERENTS ARE DEMONSTRATED BY THE FLUORESCENT TRACERS FAST BLUE AND RHODAMINE, Journal of neuroscience methods, 49(1-2), 1993, pp. 103-111
The fluorescent retrograde tracers Rhodamine B Isothiocyanate (RITC) a
nd Fast Blue (FB) were injected either into the thalamic nucleus rotun
dus or into the complexus neck muscle in pigeons. Both in the rotundal
and the peripheral motor preparations RITC yielded a 3-5 times larger
number of afferent ipsilateral neurones than FB. While RITC additiona
lly labelled a large number of contralateral afferents, virtually no l
abelled cells were detected contralateral to the injection site using
FB. This selective omission of the contralateral input with FB was ide
ntical for both neural systems despite differences in injection volume
, survival time, and transport length. In the present case, this struc
ture-specific sensitivity of FB would lead to the erroneous conclusion
that contralateral afferents are virtually non-existent in the visual
tectofugal and the peripheral motor system. Thus, these results make
it likely that the choice for a certain tracer may lead to different c
onnectional interpretations of a neural system. Although the mechanism
for these structure-specific sensitivity differences are unknown, it
is suggested that tracers with lower effectivity like FB may be unable
to label afferent structures with fewer axon terminals.