In a fattering farm for calves with clinical coccidiosis suspensions o
f oocysts were won, in which the species Eimeria zuernii predominant w
as occured with 94,0 per cent and 95,5 per cent. After the radiated at
tenuation with 250 Gy immunoprophylactical investigations were unterta
ken on a small group of calves. The application of 30 000 attenuated o
ocysts caused in comparison to fullvirulent oocysts a significant redu
ced oocysts output and showed pathological and histological a slight r
eaction on the intestine. The effectiveness of the vaccination was rev
ealed after the challenge in the output of oocysts of immunized claves
reduced to 92,2 per cent and 100 per cent comparing to non-immunized
controls.