M. Protic et As. Levine, DETECTION OF DNA DAMAGE-RECOGNITION PROTEINS USING THE BAND-SHIFT ASSAY AND SOUTHWESTERN HYBRIDIZATION, Electrophoresis, 14(8), 1993, pp. 682-692
We describe electrophoresis and biochemical conditions that allow dete
ction of damaged DNA-binding proteins in cell extracts. In addition, w
e present an overview of the damage-recognition DNA-binding proteins f
rom eukaryotic cells and discuss their hypothetical role in DNA repair
.