DETECTION OF DNA DAMAGE-RECOGNITION PROTEINS USING THE BAND-SHIFT ASSAY AND SOUTHWESTERN HYBRIDIZATION

Citation
M. Protic et As. Levine, DETECTION OF DNA DAMAGE-RECOGNITION PROTEINS USING THE BAND-SHIFT ASSAY AND SOUTHWESTERN HYBRIDIZATION, Electrophoresis, 14(8), 1993, pp. 682-692
Citations number
62
Categorie Soggetti
Biochemical Research Methods
Journal title
ISSN journal
01730835
Volume
14
Issue
8
Year of publication
1993
Pages
682 - 692
Database
ISI
SICI code
0173-0835(1993)14:8<682:DODDPU>2.0.ZU;2-C
Abstract
We describe electrophoresis and biochemical conditions that allow dete ction of damaged DNA-binding proteins in cell extracts. In addition, w e present an overview of the damage-recognition DNA-binding proteins f rom eukaryotic cells and discuss their hypothetical role in DNA repair .