PURIFICATION OF PHOSPHOENOLPYRUVATE CARBOXYLASE SUBUNITS AND ISOFORMSFROM VICIA-FABA L BY PREPARATIVE GEL-ELECTROPHORESIS AND THEIR DETECTION BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY

Authors
SCHNABL H KLOCKENBRING T HUHN M SCHULZ M
Citation
H. Schnabl et al., PURIFICATION OF PHOSPHOENOLPYRUVATE CARBOXYLASE SUBUNITS AND ISOFORMSFROM VICIA-FABA L BY PREPARATIVE GEL-ELECTROPHORESIS AND THEIR DETECTION BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY, Electrophoresis, 14(8), 1993, pp. 817-819
Citations number
10
Categorie Soggetti
Biochemical Research Methods
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
14
Issue
8
Year of publication
1993
Pages
817 - 819
Database
ISI
SICI code
0173-0835(1993)14:8<817:POPCSA>2.0.ZU;2-P
Abstract
A purification procedure which yields nearly homogenous subunits of st omatal phosphoenolpyruvate carboxylase from epidermal strips of Vicia faba L. is reported. Preparative gel electrophoresis was found to be t he most suitable technique for subunit purification. Denatured subunit s of the stomatal enzyme in the eluate were immunologically detected b y enzyme-linked immunosorbent assay (ELISA) tests. The enzyme preparat ion meets all requirements for its use in antibody production.