SIMULTANEOUS DETERMINATION OF NATIVE AND SUBUNIT MOLECULAR-WEIGHTS OFPROTEINS BY PORE LIMIT ELECTROPHORESIS AND RESTRICTED USE OF SODIUM DODECYL-SULFATE

In the present communication, we describe a method for the determinati on of both the native and subunit molecular weight of a protein by a s ingle pore limit electrophoretic run. When native proteins and sodium dodecyl sulfate (SDS)-heat denatured proteins were electrophoresed in a 4-28% gradient-polyacrylamide gel, in the presence of 0.02% SDS in r unning buffer, their respective molecular weights (native and subunit) could be determined by comparing the migration distances of unknown a nd standard proteins. The presence of SDS up to 0.02% in the running b uffer did not dissociate the native proteins into their subunits. The linearity in distribution of native and SDS-polyacrylamide gel electro phoresis (PAGE) standard marker proteins used for the molecular weight determination further showed the efficacy of the present technique.