CHROMOSOME MAPPING IN LACTIC-ACID BACTERIA

The chromosome structure of lactic acid bacteria has been investigated only recently. The development of pulsed-field gel electrophoresis (P FGE) combined with other DNA-based techniques enables whole-genome ana lysis of any bacterium, and has allowed rapid progress to be made in t he knowledge of the lactic acid bacteria genome. Lactic acid bacteria possess one of the smallest eubacterial chromosomes. Depending on the species, the genome sizes range from 1.1 to 2.6 Mb. Combined physical and genetic maps of several species are already available or close to being achieved. Knowledge of the genomic structure of these organisms will serve as a basis for future genetic studies. Macrorestriction fin gerprinting by PFGE is already one of the major tools for strain diffe rentiation, identification of individual strains, and the detection of strain lineages. The genome data resulting from these studies will be of general application strain improvement.