EFFECT OF CHOLESTEROL ADDITION ON GROWTH-KINETICS AND SHEAR-STRESS SENSITIVITY OF ADHERENT MAMMALIAN-CELLS

Authors
TOMECZKOWSKI J LUDWIG A KRETZMER G
Citation
J. Tomeczkowski et al., EFFECT OF CHOLESTEROL ADDITION ON GROWTH-KINETICS AND SHEAR-STRESS SENSITIVITY OF ADHERENT MAMMALIAN-CELLS, Enzyme and microbial technology, 15(10), 1993, pp. 849-853
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Enzyme and microbial technology → ACNP
ISSN journal
01410229
Volume
15
Issue
10
Year of publication
1993
Pages
849 - 853
Database
ISI
SICI code
0141-0229(1993)15:10<849:EOCAOG>2.0.ZU;2-M
Abstract
The growth kinetics of adherent baby hamster kidney cells cultivated w ith additional cholesterol as well as the effect of cholesterol additi on on shear stress sensitivity were investigated. The influence of var ious cholesterol preparations was tested, whereby dimethylsulfoxide an d ethanol show negative effects at higher concentrations. With additio n of cholesterol in the range of 90 mug ml-1, a positive effect on the shear stress resistance was achieved.