DIRECT DOUBLE ANTIBODY SANDWICH IMMUNOASSAY FOR PSEUDOMONAS-AERUGINOSA ELASTASE

Authors
JAFFARBANDJEE MC CARRERE J LAZDUNSKI A GUYCROTTE O GALABERT C
Citation
Mc. Jaffarbandjee et al., DIRECT DOUBLE ANTIBODY SANDWICH IMMUNOASSAY FOR PSEUDOMONAS-AERUGINOSA ELASTASE, Journal of immunological methods, 164(1), 1993, pp. 27-32
Citations number
21
Categorie Soggetti
Immunology
Journal title
Journal of immunological methods → ACNP
ISSN journal
00221759
Volume
164
Issue
1
Year of publication
1993
Pages
27 - 32
Database
ISI
SICI code
0022-1759(1993)164:1<27:DDASIF>2.0.ZU;2-N
Abstract
A direct sandwich enzyme immunoassay was developed in order to quantif y Pseudomonas aeruginosa elastase. As a solid phase the wells of a mic rotitre plate were coated with specific IgG and horseradish peroxidase labelled IgG was used as the second antibody. The detection limit of the assay was 0.26 ng/ml and a good agreement was found with elastolyt ic activity determined using elastin-Congo red. This assay was simple, specific, sensitive and reproducible, and permits the determination o f low levels of elastase.