Jt. Nakashima et al., SWEET-POTATO FEATHERY MOTTLE POTYVIRUS (C1 ISOLATE) VIRION AND RNA PURIFICATION, Journal of virological methods, 44(1), 1993, pp. 109-116
A procedure for the purification of a Peruvian isolate (C1) of sweet p
otato feathery mottle potyvirus (SPFMV) and infective RNA has been dev
eloped. The use of Hepes [N-(2-hydroxyethyl)piperazine-N'-2-ethanesulf
onic acid] buffer containing urea and sodium EDTA as a base for tissue
extraction and virus suspension enabled good yields of virus (35-50 m
g/100 g) to be obtained from Nicotiana benthamiana L. Domin. A short R
NA isolation procedure yielded infectious RNA, from which ds cDNA of n
early genome size could be obtained. Sweet potato feathery mottle poty
virus, Purification, RNA isolation, cDNA synthesis.