S. Ali et al., PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES RECOGNIZING DEFINED REGIONS OF THE HUMAN ESTROGEN-RECEPTOR, Hybridoma, 12(4), 1993, pp. 391-405
Mouse monoclonal antibodies were raised against the N-terminal (amino
acids 151-165) and the very C-terminal (amino acids 578-595) regions o
f the human oestrogen receptor (hER). These antibodies recognise the h
ER by enzyme-linked immunosorbent assay, immunocytochemistry, immunobl
otting, immunoprecipitation and gel retardation assays. The presence o
f hER is used prognostically in human breast cancer. We have tested th
e reactivity of our monoclonal antibodies on breast cancer sections, c
omparing with the commonly used Abbott rat monoclonal antibody H222. T
hese studies show that the two monoclonal antibodies described here ar
e highly versatile and will be useful tools for in vivo and in vitro s
tudies of hER function. Furthermore, we show that the corresponding ep
itopes can be used as molecular ''tags'' for heterologous proteins and
offer a powerful means of purifying and/or characterizing over-produc
ed fusion proteins containing these regions.