PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO THE CARBOXYL-TERMINAL HEPTAPEPTIDE OF ENDOTHELIN-1

Two cell lines, RR5.ET-1 and RR1.ET-1, that produce monoclonal antibod ies specific for the carboxyl-terminal heptapeptide of endothelin-1 (E T-1) have been cloned and stabilized. An RIA was developed to facilita te the evaluation and characterization of these monoclonal antibodies. The affinity constant of each MAb for ET-1, as determined by Scatchar d analysis, was 5.74 x 10(8) M-1 for RR5.ET-1 and 4.15 x 10(7) M-1 for RR1.ET-1. The antibodies reacted specifically with the carboxyl-termi nus (ET15-21) and did not cross-react with the amino-terminal amino ac ids (ET1-16). As expected, the antibodies cross-reacted with endotheli n-2 (ET-2) and endothelin-3 (ET-3), and to a lesser extent, with the c losely related sarafotoxins. Both MAbs retained about 55% reactivity w ith the ET-1 terminal sequence of Asp-Ile-Ile-Trp (ET18-21) but had no reactivity with the ET sequence His-Leu-Asp-Ile-Ile-Trp-Val-Asn (ET16 -23) nor with Big ET-1 (ET1-39). These data strongly suggest that the terminal four amino acids of ET-1 are included in the MAb binding site . More importantly, the terminal Trp21 must be free, not linked to Val 22 to retain reactivity with either of the MAbs.