NON-DIALYSIS METHOD OF RAPID AND FACILE SAMPLE PREPARATION FOR THE DESALTING AND PURIFICATION OF ENZYMES AND OTHER PROTEINS FROM PLANT-EXTRACTS

A self-contained sample preparation chromatographic device for the fac ile and rapid desalting, separation and purification of enzymes and ot her proteins is described. The method, dynamic column solid-phase extr action (DC-SPE), was applied to the extraction and separation of perox idase isozymes from crude extracts of fresh leaf tissue of three maize lines and of cucumber leaf extracts, in screening plant resistance pr operties. When packed with Sephadex G-25(M), the DC-SPE device also ga ve highly satisfactory desalting of the plant protein solutions in a m uch shorter time (10-15 min) than that required to obtain similar resu lts with Pharmacia DP-10 columns and/or by dialysis overnight.