M. Shimoni et al., NON-DIALYSIS METHOD OF RAPID AND FACILE SAMPLE PREPARATION FOR THE DESALTING AND PURIFICATION OF ENZYMES AND OTHER PROTEINS FROM PLANT-EXTRACTS, Journal of chromatography, 646(1), 1993, pp. 99-105
A self-contained sample preparation chromatographic device for the fac
ile and rapid desalting, separation and purification of enzymes and ot
her proteins is described. The method, dynamic column solid-phase extr
action (DC-SPE), was applied to the extraction and separation of perox
idase isozymes from crude extracts of fresh leaf tissue of three maize
lines and of cucumber leaf extracts, in screening plant resistance pr
operties. When packed with Sephadex G-25(M), the DC-SPE device also ga
ve highly satisfactory desalting of the plant protein solutions in a m
uch shorter time (10-15 min) than that required to obtain similar resu
lts with Pharmacia DP-10 columns and/or by dialysis overnight.