Infection with Schistosoma mansoni induces humoral and T cell mediated
responses and leads to a delayed hypersensitivity that results in gra
nulomatous inflamatory disease around the parasite eggs. Regulation of
these responses resulting in a reduction in this anti-egg inflamatory
disease is apparently determined by idiotypic repertoires of the pati
ent, associated with genetic background and multiple external factors.
We have previously reported on idiotype/anti-idiotype-receptor intera
ctions in clinical human schistosomiasis. These findings support a hyp
othesis that anti-SEA cross-reactive idiotypes develop in some patient
s during the course of a chronic infection and participate in regulati
on of anti-SEA cellular immune responses. We repport here on experimen
ts which extend those observations to the regulation of granulomatous
hypersensitivity measured by an in vitro granuloma model T cells from
chronic intestinal schistosomiasis patients were stimulated in vitro w
ith anti-SEA idiotypes and assayed in an autologous in vitro granuloma
assay for modulation of granuloma formation. These anti-SEA idiotype
reactive T cells were capable of regulating autologous in vitro granul
oma formation. Both CD4 and CD8 T cells could be activated to regulate
granuloma formation. This regulatory activity, initiated with stimula
tory anti-SEA idiotypic antibodies, was antigenically specific and was
dependent on the presence of intact (F(ab')2 immunoglobulin molecules
. The ability to elicit this regulatory activity appears to be dose de
pendent and is more easily demonstrated in chronically infected intest
inal patients or SEA sensitized individuals. These data support the hy
pothesis that anti-SEA cross reactive idiotypes are important in regul
ating granulomatous hypersensitivy in chronic intestinal schistosomias
is patients and these cross-reactive idiotypes appear to play a major
role in cell-cell interactions which result in the regulation of anti-
SEA cellular immune responses.