T. Takehara et al., INTERSPECIFIC PROTOPLAST FUSION BETWEEN S OME PLEUROTUS SPECIES USINGAUXOTROPHIC MUTANTS, Mokuzai Gakkaishi, 39(7), 1993, pp. 855-859
Interspecific protoplast fusions using auxotrophic mutants were perfor
med between three Pleurotus species, that is Pleurotus ostreatus (jacq
.: Fr.) Kummer, Pleurotus pulmonarius (Fr.) Quel., and Pleurotus cornu
copiae (Paul.) Rolla. var. citrinopileatus (Sing.) Ohira. These auxotr
ophic mutants used in this experiment were induced from monokaryotic s
trains which were isolated from the monospores of each parents. They a
re strains requiring methionine, leucine, and inositol respectively, f
or growth on minimal medium. A clamp connection was not detected in th
e hyphae of the strains obtained by protoplasts fusion in every combin
ation, and whose growth rates were considerably later than those of th
eir parents. However, both strains obtained by combinations between P.
ostreatus and P. pulmonarius, and between P. pulmonarius and P. cornu
copiae formed fruiting bodies on sawdust-wheat bran medium. These were
very similar to those of P. pulmonarius in morphology. On the other h
and, the strain obtained by protoplast fusion between P. ostreatus and
P. cornucopiae did not form even a primordium on the sawdust-wheat br
an medium.