Gm. Yakovleva et D. Dudits, EFFECTS OF AN AMINOOXY ANALOG OF PUTRESCINE ON WHEAT CELL-CULTURES (TRITICUM-MONOCOCCUM L), Journal of plant physiology, 142(2), 1993, pp. 218-221
The biochemical characterization of a non-morphogenic dedifferentiated
cell line of wheat (Triticum monococcum L.) cultured on a medium cont
aining the aminooxy analogue of putrescine, 1-aminooxy-3-aminopropane
(APA), revealed altered polyamine metabolism. APA does not inhibit the
growth of wheat cells at concentrations up to 5 mM. Exposing the call
us tissues to 0.1 mM and 1 mM APA (non-toxic concentrations) results i
n reduction of activity of both ornithine decarboxylase (ODC) and argi
nine decarboxylase (ADC) with a simultaneous increase in free putresci
ne (Put). The contents of cadaverine (Cad) and spermidine (Spd) increa
se in cultures with 0.1 mM APA as compared with the untreated callus t
issues or cultures with 1 mM APA. Neither 0.1 mM APA nor 1 mM APA caus
e significant changes in spermine (Spm) levels in the treated cells. U
nder the conditions of severe growth inhibition, APA (5 mM) completely
suppresses the ADC activity and decreases the level of free Put in th
e cell suspension culture.