EFFECTS OF AN AMINOOXY ANALOG OF PUTRESCINE ON WHEAT CELL-CULTURES (TRITICUM-MONOCOCCUM L)

Citation
Gm. Yakovleva et D. Dudits, EFFECTS OF AN AMINOOXY ANALOG OF PUTRESCINE ON WHEAT CELL-CULTURES (TRITICUM-MONOCOCCUM L), Journal of plant physiology, 142(2), 1993, pp. 218-221
Citations number
25
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
01761617
Volume
142
Issue
2
Year of publication
1993
Pages
218 - 221
Database
ISI
SICI code
0176-1617(1993)142:2<218:EOAAAO>2.0.ZU;2-E
Abstract
The biochemical characterization of a non-morphogenic dedifferentiated cell line of wheat (Triticum monococcum L.) cultured on a medium cont aining the aminooxy analogue of putrescine, 1-aminooxy-3-aminopropane (APA), revealed altered polyamine metabolism. APA does not inhibit the growth of wheat cells at concentrations up to 5 mM. Exposing the call us tissues to 0.1 mM and 1 mM APA (non-toxic concentrations) results i n reduction of activity of both ornithine decarboxylase (ODC) and argi nine decarboxylase (ADC) with a simultaneous increase in free putresci ne (Put). The contents of cadaverine (Cad) and spermidine (Spd) increa se in cultures with 0.1 mM APA as compared with the untreated callus t issues or cultures with 1 mM APA. Neither 0.1 mM APA nor 1 mM APA caus e significant changes in spermine (Spm) levels in the treated cells. U nder the conditions of severe growth inhibition, APA (5 mM) completely suppresses the ADC activity and decreases the level of free Put in th e cell suspension culture.