EPR CHARACTERIZATION OF MOLECULAR TARGETS FOR NO IN MAMMALIAN-CELLS AND ORGANELLES

Authors
HENRY Y LEPOIVRE M DRAPIER JC DUCROCQ C BOUCHER JL GUISSANI A
Citation
Y. Henry et al., EPR CHARACTERIZATION OF MOLECULAR TARGETS FOR NO IN MAMMALIAN-CELLS AND ORGANELLES, The FASEB journal, 7(12), 1993, pp. 1124-1134
Citations number
71
Categorie Soggetti
Biology,Biology
Journal title
The FASEB journal → ACNP
ISSN journal
08926638
Volume
7
Issue
12
Year of publication
1993
Pages
1124 - 1134
Database
ISI
SICI code
0892-6638(1993)7:12<1124:ECOMTF>2.0.ZU;2-Q
Abstract
Nitric oxide is synthesized in mammalian cells from L-arginine or from pharmaceutical drugs. It forms paramagnetic complexes with some metal loproteins, inhibiting key enzymes in DNA synthesis, mitochondrial res piration, iron metabolism, etc. This article reviews how electron para magnetic resonance spectroscopy helps to detect unambiguously such spe cific molecular targets for NO in mammalian whole cells and organelles . EPR has also been used for the detection of spin adducts of free NO by spin-trapping methods.