J. Zloty et al., LARVAL INSECT IDENTIFICATION BY CELLULOSE-ACETATE GEL-ELECTROPHORESISAND ITS APPLICATION TO LIFE-HISTORY EVALUATION AND COHORT ANALYSIS, Journal of the North American Benthological Society, 12(3), 1993, pp. 270-278
Early instar larvae that lack distinguishing structural characters can
be identified quickly and easily with cellulose acetate gel electroph
oresis. Examples are given from the genera Ameletus (Ephemeroptera) an
d Coenagrion, Enallagma, and Lestes (Odonata). We tested 28 or 29 enzy
mes which yielded up to 29 scorable loci; of these only six or seven w
ere necessary to achieve species separation at three or more loci. The
technique facilitates the search for structural characters, confirms
species separation based on morphology, and allows the construction of
complete life histories and life tables.