PHYLOGENETIC ANALYSIS OF BACILLUS-SPHAERICUS AND DEVELOPMENT OF AN OLIGONUCLEOTIDE PROBE SPECIFIC FOR MOSQUITO-PATHOGENIC STRAINS

Authors
DEMURO MA PRIEST FG
Citation
Ma. Demuro et Fg. Priest, PHYLOGENETIC ANALYSIS OF BACILLUS-SPHAERICUS AND DEVELOPMENT OF AN OLIGONUCLEOTIDE PROBE SPECIFIC FOR MOSQUITO-PATHOGENIC STRAINS, FEMS microbiology letters, 112(2), 1993, pp. 205-210
Citations number
13
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
112
Issue
2
Year of publication
1993
Pages
205 - 210
Database
ISI
SICI code
0378-1097(1993)112:2<205:PAOBAD>2.0.ZU;2-8
Abstract
The 16S rRNA gene sequences for six strains of Bacillus sphaericus rep resenting five distinct DNA homology groups and one strain of Bacillus fusiformis have been determined. An oligonucleotide probe based on th e area approximately between position 186 and 198 (Escherichia coli nu mbering) of the 16S rRNA gene of the mosquito pathogen, strain 2362, h ybridised to DNA from strains of DNA homology group IIA which contains all known mosquito pathogens and not to any of ten other Bacillus spe cies. It can be used to identify potential mosquito-pathogenic strains of B. sphaericus in screening programmes.