THE USE OF DAPHNIA-MAGNA FOR DETECTION OF OKADAIC ACID IN MUSSEL EXTRACTS

Authors
VERNOUX JP LEBAUT C MASSELIN P MARAIS C BARON B CHOUMILOFF R PRONIEWSKI F NIZARD G BOHEC M
Citation
Jp. Vernoux et al., THE USE OF DAPHNIA-MAGNA FOR DETECTION OF OKADAIC ACID IN MUSSEL EXTRACTS, Food additives and contaminants, 10(5), 1993, pp. 603-608
Citations number
NO
Categorie Soggetti
Food Science & Tenology","Chemistry Applied","Public, Environmental & Occupation Heath
Journal title
Food additives and contaminants → ACNP
ISSN journal
0265203X
Volume
10
Issue
5
Year of publication
1993
Pages
603 - 608
Database
ISI
SICI code
0265-203X(1993)10:5<603:TUODFD>2.0.ZU;2-O
Abstract
Okadaic acid (OA), the main toxin responsible for diarrhetic shellfish poisoning (DSP) has toxicity for Daphnia magna (EC50 = 15 +/- 1.8 mug /l). A Daphnia bioassay was developed and used to analyse okadaic acid in mussel extracts. A linear correlation was found between OA concent ration determined by the Daphnia bioassay and by HPLC assay (r = 0.85; p < 0.001). The Daphnia bioassay can measure OA levels 10 times below the threshold of the mouse bioassay method. It is an inexpensive sens itive tool which can be used in replacement of mouse bioassay for the screening of OA and some co-extracting toxins in mussel extracts.