Rw. Silman et al., PRODUCTION OF COLLETOTRICHUM-TRUNCATUM FOR USE AS A MYCOHERBICIDE - EFFECTS OF CULTURE, DRYING AND STORAGE ON RECOVERY AND EFFICACY, Biotechnology advances, 11(3), 1993, pp. 561-575
Colletotrichum truncatum (Schwein.) Andrus and Moore NRRL 13737 (= NRR
L 18434) is a fungal plant pathogen which shows promise as a bioherbic
ide against the troublesome weed Sesbania exaltata (Raf.) Rydb. ex A.
W. Hill. previous studies showed similar amounts of spores were produc
ed/ml of medium in liquid and solid-state fermentations. In this study
, Colletotrichum truncatum spores were produced in liquid (LC), solid/
vermiculite (SV), and solid/perlite-cornmeal-agar (SP). After drying a
t room temperature with flowing air, SV and SP retained the most viabi
lity. Each product was then stored at 4-degrees, 15-degrees, and 25-de
grees-C. All three products stored at 4-degrees-C and SP stored at 15-
degrees-C retained highest viability. Efficacy based upon assays utili
zing numbers of viable spores showed SV and SP spores incited more sev
ere disease symptoms than LC spores.