Rd. Tanner et al., REMOVAL OF FIBER FROM VINES BY SOLID-STATE FERMENTATION ENZYMATIC DEGRADATION - A COMPARISON OF FLAX AND KUDZU RETTING, Biotechnology advances, 11(3), 1993, pp. 635-643
Kinetic data describing the decomposition of the outer sheath of kudzu
vines (undergoing a solid fermentation process in a glass beaker of s
oil) have been analyzed to determine the two constants, K(m)/S(o) and
V/S(o), where S(o) is the initial substrate concentration, K(m) the Mi
chaelis constant, and V the maximum product rate. The kinetic data are
expressed by a simple time-varying desheathing index, obtained from t
he number of spatula scrapings required to reach the desired hard cell
ulosic fibers (vascular bundles) of the plant. A simple relationship b
etween the desheathing index, D.I. and the dimensionless product conce
ntration, P/S(o) is proposed to relate the D.I. data and enzyme kineti
c concentration data. Thus, the Michaelis-Menten enzyme kinetic parame
ters can be estimated from easily obtained physical (non-chemical data
; the D.I.(t) measurements). This low energy process for processing vi
nes into valuable fibers is similar to the traditional microbial metho
d for recovering flax fibers for linen cloth, by retting of the flax p
lant vines, except there is no unbound liquid water present in the soi
l medium.