A HIGH-DENSITY STS MAP-BASED ON A SINGLE CONTIG OF YAC AND P1 CLONES IN THE CHROMOSOME 8P12-P21 REGION

We have constructed a yeast artificial chromosome (YAC) and P1 contig in the 8p12-p21 region. The contig comprises 16 overlapping YAC clones and 44 overlapping P1 clones. Twelve dinucleotide-repeat polymorphic sequence-tagged site (STS) markers that were previously isolated mainl y from these YAC and P1 clones were genetically mapped. A total of 46 nonpolymorphic STS markers were newly established mainly from the YAC and P1 clone end fragments, and 28 of the 46 nonpolymorphic STSs, as w ell as the 12 polymorphic STSs, were also mapped physically onto the c ontig based on STS content analysis of YAC pools and of the P1 and YAC clones. As a result, the YAC and P1 clones were assembled into a sing le contig covering a minimum of 1.5 Mb physically and 2.8 cM genetical ly with 12 polymorphic and 28 nonpolymorphic STSs within the 8p12-p21 region. Average STS spacing in the contig was estimated to be 40 kb/ST S. In addition, further characterization of the contig suggested that this contig includes a region where genetic recombination occurs frequ ently. Thus, the resulting cloned region, together with densely mapped STS markers on the contig, should help to promote our understanding o f this region. (C) 1997 Academic Press.