CD156 (HUMAN ADAM8) - EXPRESSION, PRIMARY AMINO-ACID-SEQUENCE, AND GENE LOCATION

The murine cell-surface antigen MS2 [ADAM 8; mouse CD156 (mCD156)] is a transmembrane glycoprotein that is highly expressed in monocytic lin eages. mCD156 consists of a long exterior region containing domains st rikingly similar to those of hemorrhagic snake venom proteins. cDNA fo r human CD156 was isolated from cDNA libraries from the human macropha ge cell line THP-1 and from human granulocytes. The CD156 cDNA detecte d mRNA from human macrophage cell lines, granulocytes, monocytes, and B cell but not T cell lines, The nucleotide sequence of the CD156 cDNA showed 65.6% homology with that of mCD156, and its amino acid sequenc e had high homology with hemorrhagic snake venom proteins and other re lated mammalian proteins, The CD156 gene was mapped to human chromosom e 10q26.3. (C) 1996 Academic Press.