Hpm. Horikawa et al., A COMPLEX OF RAB3A, SNAP-25, VAMP SYNAPTOBREVIN-2 AND SYNTAXINS IN BRAIN PRESYNAPTIC TERMINALS/, FEBS letters, 330(2), 1993, pp. 236-240
Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-
type calcium channels. On immunoaffinity chromatography of digitonin e
xtracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, prote
ins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained
by both columns. Here we show that the 19 and 28 kDa bands contain VA
MP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since
SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/sm
g25A and SNAP-25), respectively, the results indicate that all these p
roteins form a complex. Our results suggest tight linkage between the
components involved in neurotransmitter release.