A COMPLEX OF RAB3A, SNAP-25, VAMP SYNAPTOBREVIN-2 AND SYNTAXINS IN BRAIN PRESYNAPTIC TERMINALS/

Authors
HORIKAWA HPM SAISU H ISHIZUKA T SEKINE Y TSUGITA A ODANI S ABE T
Citation
Hpm. Horikawa et al., A COMPLEX OF RAB3A, SNAP-25, VAMP SYNAPTOBREVIN-2 AND SYNTAXINS IN BRAIN PRESYNAPTIC TERMINALS/, FEBS letters, 330(2), 1993, pp. 236-240
Citations number
27
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
330
Issue
2
Year of publication
1993
Pages
236 - 240
Database
ISI
SICI code
0014-5793(1993)330:2<236:ACORSV>2.0.ZU;2-4
Abstract
Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N- type calcium channels. On immunoaffinity chromatography of digitonin e xtracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, prote ins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VA MP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/sm g25A and SNAP-25), respectively, the results indicate that all these p roteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.