KINETIC EFFECT OF THE SUBSTRATE INSTABILITY ON THE MICHAELIS-MENTEN MECHANISM

Authors
DELSOLO CG CASTELLANOS RV CANOVAS FG HAVSTEEN BH
Citation
Cg. Delsolo et al., KINETIC EFFECT OF THE SUBSTRATE INSTABILITY ON THE MICHAELIS-MENTEN MECHANISM, Anales de quimica, 89(3), 1993, pp. 319-324
Citations number
12
Categorie Soggetti
Chemistry
Journal title
Anales de quimica → ACNP
ISSN journal
11302283
Volume
89
Issue
3
Year of publication
1993
Pages
319 - 324
Database
ISI
SICI code
1130-2283(1993)89:3<319:KEOTSI>2.0.ZU;2-4
Abstract
A complete kinetic analysis of Michaelis-Menten mechanism, in which th e substrate is unstable, is presented. This analysis allows the deriva tion of equation which relate the concentration of the product of the enzyme reaction to the time. A kinetic data analysis for the evaluatio n of all of the individual rate constants of the reactions is suggeste d. It is based on the time course equation and the experimental progre ss curve of the product.