A complete kinetic analysis of Michaelis-Menten mechanism, in which th
e substrate is unstable, is presented. This analysis allows the deriva
tion of equation which relate the concentration of the product of the
enzyme reaction to the time. A kinetic data analysis for the evaluatio
n of all of the individual rate constants of the reactions is suggeste
d. It is based on the time course equation and the experimental progre
ss curve of the product.